Motor behavior represents the ultimate output of most nervous system activity. Its accuracy
depends on precise connectivity of many different circuit modules, together controlling programs
for motor output, computing predicted action and monitoring consequences of past action. Body
movement entails the concerted activation of limb muscles innervated by specialized groups of
spinal motor neurons, yet very little is known about synaptic organization and function of
connectivity modules to diverse motor neurons, at the core of regulating motor output programs.
This talk will focus on our recent work unraveling organizational and functional principles of
neuronal circuits regulating the control of spinal motor neurons innervating limb muscles.
Exploiting transsynaptic virus tools, mouse genetics and behavioral experiments, we found striking
differences in anatomical and functional organization of direct connections to different motor
neuron pools by distinct classes of spinal interneurons and supraspinal brainstem nuclei. Our
studies on organization and function of descending brainstem command lines point towards the
existence of segregated action maps to allow motor program diversification at the level of the
brainstem, just one synapse away from motor neurons. Together, our findings demonstrate that
functional differences discerned at the motor output level and important for appropriate motor
behavior are engraved anatomically and genetically as differential spatial maps at the premotor
level, both at spinal and supraspinal levels