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DTSTART:20170326T030000
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DTSTART:20161030T020000
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BEGIN:VEVENT
DTSTAMP:20260428T232956Z
UID:585bf2886581f186732030@ist.ac.at
DTSTART:20170116T084500
DTEND:20170116T094500
DESCRIPTION:Speaker: Doreen Matthies\nAbstract: Cryo-EM is currently revolu
 tionizing the field of structural biology. Due to recent advances in detec
 tor technologies\, hardware & software improvements\, this method is becom
 ing more and more mainstream in solving high-resolution structures. It com
 plements methods such as X-ray crystallography & NMR spectroscopy with the
  advantage of not having to form well-ordered crystals or having an upper 
 size limit. Progress in the field of single-particle cryo-EM will be discu
 ssed with examples of soluble & membrane protein complexes yielding resolu
 tions of better than 3 Å. A study on 200 kDa Mg2+ channel CorA will be us
 ed as a detailed example. CorA is a homo-pentamer and is gated by intracel
 lular Mg2+. We determined the structures of CorA in the presence and absen
 ce of Mg2+ using single-particle cryo-EM. The five-fold symmetric Mg2+-bou
 nd closed state of CorA at an overall 3.8 Å resolution reveals side-chain
  densities as well as densities for several magnesium ions. Cryo-EM of Mg2
 +-free open state CorA revealed dramatic differences in the conformation o
 f the channel with large asymmetric changes in the soluble domain of the p
 entameric complex suggesting an unprecedented gating mechanism.
LOCATION:Mondi Seminar Room 2\, Central Building\, ISTA
ORGANIZER:pdelreal@ist.ac.at
SUMMARY:Doreen Matthies: Single-Particle Cryo-EM: A rising star in structur
 al biology
URL:https://talks-calendar.ista.ac.at/events/97
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