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DTSTART:20170326T030000
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DTSTART:20171029T020000
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DTSTAMP:20260427T115952Z
UID:59b6ac059ccef825061984@ist.ac.at
DTSTART:20170915T143000
DTEND:20170915T153000
DESCRIPTION:Speaker: Patrick Oakes\nhosted by Michael Sixt\nAbstract: The a
 bility of adherent cells to sense changes in the mechanical properties of 
 their extracellular environments is critical to numerous aspects of their 
 physiology. It has been well documented that cell attachment and spreading
  are sensitive to substrate stiffness. Here we demonstrate that this behav
 ior is actually biphasic\, with a transition that occurs around a Young’
 s modulus of ~7 kPa. Furthermore\, we demonstrate that\, contrary to estab
 lished assumptions\, this property is independent of myosin II activity. R
 ather\, we find that cell spreading on soft substrates is inhibited due to
  reduced nascent adhesion formation within the lamellipodium. Cells on sof
 t substrates display normal leading edge protrusion activity\, but these p
 rotrusions are not stabilized due to impaired adhesion assembly. Enhancing
  integrin-ECM affinity through addition of Mn2+ recovers nascent adhesion 
 assembly and cell spreading on soft substrates. Using a computational mode
 l to simulate nascent adhesion assembly\, we find that biophysical propert
 ies of the integrin-ECM bond are optimized to stabilize interactions above
  a threshold matrix stiffness that is consistent with the experimentally o
 bservations. Together these results suggest that myosin II-independent for
 ces in the lamellipodium are responsible for mechanosensation by regulatin
 g new adhesion assembly\, which in turn\, directly controls cell spreading
 .  This myosin II-independent mechanism of substrate stiffness sensing co
 uld potentially regulate a number of other stiffness sensitive processes.
LOCATION:Seminar Room\, Lab Building East\, ISTA
ORGANIZER:astawick@ist.ac.at
SUMMARY:Patrick Oakes: A Myosin Independent Mechanism of Mechanosensing
URL:https://talks-calendar.ista.ac.at/events/822
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