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DTSTART:20260329T030000
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DTSTAMP:20260709T063031Z
UID:1785744000@ist.ac.at
DTSTART:20260803T100000
DTEND:20260803T110000
DESCRIPTION:Speaker: Marek Randuch\nhosted by Michael Sixt\nAbstract: Root 
 growth inhibition (RG) by auxin and other cues (e.g. eATP\, RALF peptide) 
 involves rapid\, non-transcriptional pathways. Specific steps leading to g
 rowth inhibition are unclear. Notably\, the RG inhibition is associated wi
 th increases in cytosolic Ca2+\, plasma membrane depolarization and apopla
 st alkalization. However\, the causal relationship between them is unclear
 . To untangle these processes\, we utilized multiplexed reporter imaging 
 to observe pairwise the real-time dynamics of these three processes. Furth
 ermore\, we tested causal relationships. We employed microfluidics for rap
 id medium exchange and leveraged new optogenetically regulated channels. 
 Our comprehensive approach uncovered a network with tight feedback loops. 
 Results indicate that cytosolic Ca2+ serves as the universal initial signa
 l\, triggering both plasma membrane depolarization and subsequent apoplast
  alkalization following auxin perception. his conclusion provides a basis 
 for the fundamental regulatory mechanism governing growth but also opens q
 uestions about the molecular mechanisms behind it.
LOCATION:Office Bldg West / Ground floor / Heinzel Seminar Room (I21.EG.101
 ) and Zoom\, ISTA
ORGANIZER:
SUMMARY:Marek Randuch: Thesis Defense: Fast growth responses in plants: Pho
 spho‐proteomic and growth regulation
URL:https://talks-calendar.ista.ac.at/events/6553
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