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DTSTART:20260329T030000
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DTSTAMP:20260424T143500Z
UID:699c104cded21642660514@ist.ac.at
DTSTART:20260415T110000
DTEND:20260415T120000
DESCRIPTION:Speaker: Filippo Conforto\nhosted by Andela Saric\nAbstract: Th
 e activity and organisation of DNA in the cell are highly regulated by sev
 eral proteins\, such as topoisomerases and Structural Maintenance of Chrom
 osomes (SMCs)\, which are involved in topological regulation by resolving 
 crossings between DNA strands or creating loops within chromatin. While we
  have rich knowledge about the impact of these proteins on the cell's func
 tion\, the rheological\, i.e.\, flow properties\, which affect the capacit
 y of DNA to reorganise and respond to stimuli\, are still poorly understoo
 d.In this talk\, I will investigate the viscous and elastic properties of 
 dense DNA solutions under the effect of SMCs and the formation of gels thr
 ough DNA ligation. Specifically\, I show how SMCs modulate in silico and i
 n vitro the rheological properties both through the extrusion of loops and
  the creation of transient crosslinks between DNA strands. Additionally\, 
 I investigate how DNA can be used to create percolating networks of linked
  rings. These DNA networks\, called "Olympic Gels''\, differ from classic 
 transiently linked gels by the presence of permanent topological links. I 
 show that through progressive ligation it is possible to create materials 
 with tuneable viscoelastic properties\, which can be controlled by choosin
 g the length of ring and linear DNA strands used in the gel formation. Thi
 s work contributes to a better understanding of how proteins naturally con
 tained in the cell affect the topology\, structure\, and rheology of entan
 gled DNA\, and will help guide the design of new biomaterials inspired by 
 the properties of DNA.
LOCATION:Sunstone Bldg / Ground floor / Big Seminar Room A / 27 seats (I23.
 EG.102)\, ISTA
ORGANIZER:mmunozba@ist.ac.at
SUMMARY:Filippo Conforto: Modulating the flow of entangled DNA with topolog
 ically active proteins
URL:https://talks-calendar.ista.ac.at/events/6381
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