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DTSTART:20260329T030000
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BEGIN:VEVENT
DTSTAMP:20260416T222501Z
UID:1771578000@ist.ac.at
DTSTART:20260220T100000
DTEND:20260220T110000
DESCRIPTION:Speaker: Fidel Emmanuel Serrano\nhosted by Alicia Michael\nAbst
 ract: The mammalian circadian clock is governed by a feedback loop in whic
 h the transcription activator CLOCK:BMAL1 induces expression of its inhibi
 tors\, PERs and CRYs\, which form a complex with CK1δ\, the main circadia
 n kinase. However\, the spatiotemporal dynamics of this feedback loop and 
 the precise role of CK1δ remain incompletely understood. Using an inducib
 le overexpression system\, we show that nuclear availability of CK1δ is l
 imited by both rapid nuclear degradation and active export of unassembled 
 kinase\, while cytoplasmic kinase is readily available for association wit
 h PERs. We demonstrate that CK1δ-mediated phosphorylation may disrupt PER
 2-CRY1 interaction thereby resulting in cytoplasmic PER2 dimers containing
  substoichiometric amounts of CRY1. Analysis of endogenous PER2 localizati
 on in the context of an intact circadian clock reveals that PER2 accumulat
 es in the cytoplasm late in the circadian cycle. Based on these findings\,
  we propose that cytoplasmic accumulation of PER:CRY:CK1δ complexes contr
 ibutes to the clearance of nuclear PER2\, while the CK1δ-dependent releas
 e of CRY1 into the nucleus may sustain CLOCK:BMAL1 repression on DNA suppo
 rting the transition from the early to the late repressive phase.
LOCATION:Moonstone Bldg / Ground floor / Seminar Room E\, ISTA
ORGANIZER:alicia.michael@ista.ac.at
SUMMARY:Fidel Emmanuel Serrano: Nuclear CK1δ as a Critical Determinant of 
 PER:CRY Complex Dynamics and Circadian Period 
URL:https://talks-calendar.ista.ac.at/events/6291
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