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DTSTAMP:20260424T040551Z
UID:1758625200@ist.ac.at
DTSTART:20250923T130000
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DESCRIPTION:Speaker: Michaela Misova\nhosted by Johann Danzl\nAbstract: Neu
 rons communicate with each other via chemical and electrical synapses. Whi
 le much attention has been devoted to understanding the molecular players 
 involved in chemical neurotransmission\, the biology of gap junctions medi
 ating electrical communication is understudied. This imbalance is not for 
 their lack of significance – electrical synapses make up about 20% of ne
 uronal connections in different species from invertebrates to vertebrates\
 , but rather reflects technical constraints and investigator bias. Many re
 gard electrical synapses as simple channels\, and much of what is known ab
 out them comes from studies on cell culture models. Insights into their in
  vivo biology are just starting to emerge. The channels of electrical syn
 apses in most animals are formed by innexins. Chordates are an exception: 
 their electrical synapses consist of connexins. Even though at the level o
 f primary sequence innexins and connexins appear to be unrelated\, structu
 rally and functionally they have much in common. The nematode Caenorhabdit
 is elegans is a promising model to study the biology of electrical synapse
 s. This animal offers a powerful toolbox to study neuron function at the m
 olecular\, cell biological and physiological levels. It also offers a deta
 iled connectome map of all of its 302 neurons. The implications from studi
 es of molecular pathways in C. elegans go beyond this organism\, since ~80
 % of the nematode’s proteins have human homologs. This work aims to enh
 ance our understanding of the in vivo biology of gap junctions using C. el
 egans as a model. Using a proximity labeling approach\, we profiled the in
 teractomes of two innexins that are expressed predominantly in neurons: UN
 C-7 and UNC-9. We identified scores of proteins that may have important ro
 les for these innexins and their electrical synapses. Using localization a
 nd functional assays to validate and further probe our findings\, both at 
 the whole organism and the single-cell level\, we found that gap junctions
  have heterogeneous protein compositions. Our findings imply that electric
 al synapses are sophisticated\, molecularly diverse structures. 
LOCATION:Central Bldg / O1 / Mondi 2b (I01.O1.008) and Zoom\, ISTA
ORGANIZER:
SUMMARY:Michaela Misova: Thesis Defense: Dissecting gap junction biology us
 ing the C. elegans nervous system
URL:https://talks-calendar.ista.ac.at/events/5984
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