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DTSTAMP:20260424T040536Z
UID:1756882800@ist.ac.at
DTSTART:20250903T090000
DTEND:20250903T100000
DESCRIPTION:Speaker: Kasumi Kishi\nhosted by Mario de Bono\nAbstract: Durin
 g vertebrate development\, the morphogen Sonic Hedgehog (Shh) plays a key 
 role in patterning the tissues of the extending body. This requires precis
 e coordination between the size of the source of Shh production and the em
 bryo body. Shh is produced by the notochord\, as well as floorplate of the
  neural tube. In this thesis\, we investigated how the sizes of the notoch
 ord and floorplate are regulated and how they impact the developing mouse 
 embryo. To measure the size and shape of the developing mouse notochord a
 nd the cell behaviours within this organ\, we performed high-resolution me
 asurements in cleared embryos between E8.5 and E10.5 of development. Strik
 ingly\, our data revealed that the notochord maintains a constant cross-se
 ctional area throughout the trunk over time as it lengthens by cell prolif
 eration. We show that this is achieved by a feedback between the notochord
  cell division rate and the elongation of the embryo. Using live imaging\,
  we further show that notochord cells undergo posteriorly-directed active 
 cell migration and that this cell migration contributes to maintaining the
  structural integrity of the notochord. Our results show a complex coordin
 ation of cell behaviours that allow for robust notochord morphogenesis dur
 ing mouse development and the maintenance of a defined Shh source size. S
 hh produced by the notochord induces a secondary Shh-producing domain\, th
 e floor plate. How the notochord and floor plate contribute to the formati
 on of the Shh gradient in the neural tube and to the regulation of the flo
 or plate size is poorly understood. Our theoretical and experimental analy
 sis indicates that only the initial levels of notochord-derived Shh influe
 nce the size of the floor plate\, and the floor plate is insensitive to it
 s own production of Shh. After its initial establishment\, the floor plate
  expands by tissue growth\, leading to scaling of the Shh gradient amplitu
 de with tissue size.  Altogether\, we show that the mechanisms regulatin
 g the sizes of the notochord and floorplate in mouse development help to a
 chieve size robustness of these tissues. Our data demonstrate that the ini
 tial establishment of the Shh gradient in the neural tube is strongly depe
 ndent on the notochord and suggest that the robustness of notochord morpho
 genesis is essential for pattern formation. Later in development\, the flo
 or plate size and the Shh gradient are coupled to neural tube growth. Our 
 findings provide new insights on how pattern formation\, tissue growth and
  morphogenesis interact to ensure reproducible developmental outcomes.
LOCATION:Central Bldg / O1 / Mondi 2a (I01.O1.008) and Zoom\, ISTA
ORGANIZER:
SUMMARY:Kasumi Kishi: Thesis Defense: Regulation of notochord and floor pla
 te size during mouse development
URL:https://talks-calendar.ista.ac.at/events/5968
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