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DTSTAMP:20260424T143331Z
UID:1755518400@ist.ac.at
DTSTART:20250818T140000
DTEND:20250818T150000
DESCRIPTION:Speaker: Osvaldo Miranda Romero\nhosted by Sandra Siegert\nAbst
 ract: Cortical development has been studied for many decades and with each
  passing year\, we learn more about the processes that govern the tightly 
 regulated developmental program. Up until recently\, the field has struggl
 ed to gain reliable access to progenitors at the single-cell level\, limit
 ing the resolution at which we can study cortical development in vivo. The
 refore\, much of the data we have on mammalian cortical development is at 
 the cell population level. The subsequent pages of this thesis describe no
 vel elements of cortical development in mice\, acquired by using a candida
 te gene approach in conjunction with Mosaic Analysis with Double Markers (
 MADM) to achieve single cell resolution. In this thesis\, I briefly provid
 e an overview of the state of the field\, describe the methodology used to
  quantify morphological characteristics of neurons and glia\, and delve in
 to the role of Pten in neurogenesis and gliogenesis in the developing mous
 e cortex. By using MADM to generate genetic mosaics\, in which only a smal
 l fraction of cells are mutated\, to study population-level changes\, we c
 an bypass the early postnatal lethality reported in Pten conditional knock
 outs (cKOs) using Emx1-Cre. Pten-mutant neuron populations within the mosa
 ics were significantly expanded. We used Emx1-CreERT2 to induce clonal del
 etion of Pten\, and quantify RGP neurogenic output at the single progenito
 r level. In the latter parts of this thesis\, we described how Pten deleti
 on leads to an increase in the abundance of astrocytes. We availed ourselv
 es of the flexibility offered by the MADM system to generate Pten mosaics 
 within a series of distinct knockout tissues to test for the interactions 
 between Pten and a battery of genes involved in several pro-gliogenic sign
 aling pathways. Our survey of Pten’s interactions with genes in key sign
 aling pathways reveals that Erk1/2 are the critical effectors downstream o
 f EGFR signaling essential for gliogenesis. Our results demonstrate that P
 ten plays critical and distinct roles throughout cortical RGP lineage prog
 ression.
LOCATION:Office Bldg West / Ground floor / Heinzel Seminar Room (I21.EG.101
 ) and Zoom\, ISTA
ORGANIZER:
SUMMARY:Osvaldo Miranda Romero: Thesis Defense: Unraveling the Role of Pten
  in Cortical Stem Cell Lineage Progression using MADM
URL:https://talks-calendar.ista.ac.at/events/5942
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