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DTSTART:20170326T030000
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DTSTART:20171029T020000
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DTSTAMP:20260428T112241Z
UID:58f09cdbab50a871791308@ist.ac.at
DTSTART:20170418T140000
DTEND:20170418T150000
DESCRIPTION:Speaker: Albert Guskov\nhosted by Leonid Sazanov\nAbstract: Zin
 c is an essential microelement to sustain all forms of life. However exces
 s of zinc is toxic\, and therefore organisms have developed complex system
 s for tight regulation of the zinc concentration using dedicated proteins 
 for import\, export and storage of zinc. In Enterobacteriaceae several mem
 brane transporters are involved in zinc homeostasis and linked to virulenc
 e: the ABC transporter ZnuABC and ZIP-family transporter ZupT for import\;
  and P-type ATPase ZntA and cation-diffusion facilitator YiiP transporter 
 for export.  In addition ZntB\, a member of the MIT family\, has been repo
 rted to be involved in the export of zinc\, but the mechanism of zinc tran
 sport by ZntB is poorly understood and based mostly on experimental charac
 terization of its distant homologue CorA\, which is a magnesium channel. \
 nIn this talk I will describe the cryo-electron microscopy structure of fu
 ll-length ZntB from Escherichia coli together with the results of isotherm
 al titration calorimetry\, radio-ligand uptake and fluorescent transport a
 ssays on ZntB reconstituted into liposomes. Our results clearly show that 
 ZntB mediates Zn2+ uptake\, stimulated by a pH gradient across the membran
 e\, using a transport mechanism that does not resemble the one recently pr
 oposed by Matthies et al. for homologous CorA channels.
LOCATION:Seminar Room\, Lab Building East\, ISTA
ORGANIZER:ddomniso@ist.ac.at
SUMMARY:Albert Guskov: Cryo-EM and functional studies on zinc transporter Z
 ntB
URL:https://talks-calendar.ista.ac.at/events/574
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