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DTSTAMP:20260424T143434Z
UID:1732266000@ist.ac.at
DTSTART:20241122T100000
DTEND:20241122T114000
DESCRIPTION:Speaker: Martin Obr\nhosted by Alicia Michael & Florian Schur\n
 Abstract: Data processing for cryo-electron tomography (cryo-ET)\, while r
 ooted in the principles of single particle analysis (SPA)\, presents uniqu
 e challenges\, pitfalls\, but also advantages. The tilt series used for to
 mogram reconstruction comprises low-dose\, low signal-to-noise ratio (SNR)
  images\, often impacted by significant drift caused by stage tilting. At 
 the same time\, feature-rich cryo-ET samples\, such as FIB-milled lamellae
 \, offer abundant signal for robust motion correction and CTF fitting. Pro
 perly harnessing this potential during subtomogram averaging can help achi
 eving higher resolutions.A critical yet often overlooked aspect of cryo-ET
  processing is ensuring the correct handedness of reconstructed volumes. U
 nlike SPA\, cryo-ET inherently preserves absolute handedness throughout im
 age processing. However\, achieving this requires careful navigation of mi
 croscope\, detector\, and reconstruction conventions—challenges compound
 ed by the lack of universal standardization. Confidence in data processing
  accuracy remains the foundation of reliable cryo-ET results. Moving forwa
 rd\, rigorous validation protocols and unified conventions will be essenti
 al for advancing cryo-ET as a mature structural biology technique\, unique
 ly capable of resolving biomolecular structures in their native environmen
 ts.
LOCATION:Moonstone Ground Floor Seminar Room E\, ISTA
ORGANIZER:alicia.michael@ista.ac.at 
SUMMARY:Martin Obr: Freeze! Show Your Hands… Apprehending Motion and Hand
 edness to Get the Most from Cryo-Electron Tomography
URL:https://talks-calendar.ista.ac.at/events/5422
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