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DTSTART:20120325T030000
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DTSTAMP:20260506T042301Z
UID:5004044d119a4@ist.ac.at
DTSTART:20120130T163000
DTEND:20120130T173000
DESCRIPTION:Speaker: Valentina Emiliani\nAbstract: The combination of light
  microscopy and optogenetics actuators and reporters offers the\npossibili
 ty to control activation and inhibition of neuronal activity and monitor f
 unctional\nresponses in a non-invasive manner enabling the analysis of wel
 l-defined neuronal population\nwithin intact neuronal circuits and systems
 . Interestingly\, those tools have permitted to address\nkey biological qu
 estions with relatively simple illumination methods using widefield visibl
 e light\nillumination. However\, some limitations in the specificity of ge
 netic targeting and the intricate\nmorphology of the brain (neuronal proce
 sses\, such as dendrites and axons\, can reach regions far\naway from the 
 cell soma)\, make it challenging to\, for example\, individuate subsets of
  genetically\nidentical interconnected cells\, or to establish the role of
  specific spatiotemporal excitatory\npatterns in guiding animal behavior. 
 To reach such degree of specificity\, more sophisticated\nillumination met
 hods are required\, permitting control of light patterning deep inside tis
 sues.\nHere we present a novel microscopy system for high-resolution patte
 rned photoactivation of\noptogenetics molecules based on the temporal cont
 rol of ultrafast pulses for axial localization of\nthe illumination patter
 n. For lateral light patterning\, the system can use either digital hologr
 aphy\n[1-3] or the generalized phase contrast method [4].\nWe apply sculpt
 ed two-photon illumination to activate Channelrhodopsin-2 in mouse culture
 d\nneurons and cortical slices with sufficient efficacy to reliably fire a
 ction potentials with millisecond\ntemporal resolution and low excitation 
 power when the light was shaped over the cell body\, one\nor more dendriti
 c subdomains or multiple cells simultaneously [4].
LOCATION:Raiffeisen Lecture Hall\, Central Building\, ISTA
ORGANIZER:ihetzenauer@ist.ac.at
SUMMARY:Valentina Emiliani: The Institute Colloquium: Two-photon optogeneti
 cs
URL:https://talks-calendar.ista.ac.at/events/448
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