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UID:1693483200@ist.ac.at
DTSTART:20230831T140000
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DESCRIPTION:Speaker: Joerg Bewersdorf\nhosted by Johann Danzl\nAbstract: Su
 per-resolution optical microscopy has become a powerful tool to study the 
 nanoscale spatial distribution of molecules of interest in biological cell
 s\, tissues and other structures over the last years. Imaging these distri
 butions in the context of other molecules or the general structural contex
 t is\, however\, still challenging. I will present two recent developments
  from my lab that tackle this challenge: first\, pan-Expansion Microscopy
  is a new sample preparation technique which expands a fixed cell or tiss
 ue sample physically by about a factor of 20 in all three dimensions\, the
 reby making small structures resolvable with just a standard confocal micr
 oscopes. Since most proteins are retained in our expansion process\, prote
 ins and other cellular components can be labeled in bulk. This provides ul
 trastructural context to the nanoscale organization of proteins and thereb
 y presents an all-optical imaging alternative to complex correlative light
 /electron microscopy [1\,2\,3]. Second\, FLASH-PAINT introduces a novel 
 type of fluorescent labels that allow for spectrally unlimited multiplexed
  imaging (super-resolution or conventional) in a rapid\, highly efficient\
 , and gentle way without any need for washing steps [4\,5]. It allows supe
 r-resolution imaging of more than 10 labels in the same sample and can equ
 ally be applied in spatial omics applications with the potential to image 
 hundreds of markers.Financial Interest Disclosure: J.B. is co-founder of p
 anluminate\, a startup company related to Expansion Microscopy.[1] M’Saa
 d\, O.\, Bewersdorf\, J. “Light microscopy of proteins in their ultrastr
 uctural context”. Nature Communications (2020). https://doi.org/10.1038/
 s41467-020-17523-8[2] M’Saad\, O.\, et al. “All-optical visualization 
 of specific molecules in the ultrastructural context of brain tissue”. b
 ioRxiv (2022). https://doi.org/10.1101/2022.04.04.486901[3] M’Saad\, O.\
 , et al. “Unclearing Microscopy”. bioRxiv (2022). https://doi.org/10.1
 101/2022.11.29.518361[4] Chung\, K.K.H. et al. “Fluorogenic DNA-PAINT fo
 r faster\, low-background super-resolution imaging”. Nature Methods (202
 2). https://doi.org/10.1038/s41592-022-01464-9[5] Schueder\, F.\, et al. 
 “Unraveling cellular complexity with unlimited multiplexed super-resolut
 ion imaging”. bioRxiv (2023)
LOCATION:Mondi 2\, Central Building\, ISTA
ORGANIZER:omerhalil.unal@ist.ac.at
SUMMARY:Joerg Bewersdorf: Super-resolution Microscopy Developments for Tomo
 rrow's Cell Biology
URL:https://talks-calendar.ista.ac.at/events/4378
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