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DTSTAMP:20260424T040533Z
UID:1713173400@ist.ac.at
DTSTART:20240415T113000
DTEND:20240415T123000
DESCRIPTION:Speaker: Andrew G. Clark\nhosted by Beatriz Vicoso\nAbstract: A
 bstractDiploid organisms have two copies of most genes\, one from the moth
 er and one from the father.  When it comes to gene expression\, the two o
 ften deviate from equal expression\, and there can be profound evolutionar
 y consequences of this.  Phenomena like dominance and genomic imprinting 
 are two such examples of molecular mechanisms that result in a departure f
 rom simple additive effects of alleles. Transvection is a genetic phenomen
 on whereby a heterozygote for two different defective alleles may have wil
 d-type function. While transvection has been amply demonstrated at a handf
 ul of genetic loci in Drosophila (e.g. bicoid\, yellow)\, an outstanding q
 uestion in genetics is whether it occurs at all genes. A more general form
  of the question is to ask – “Is the expression level of an allele str
 ictly a property of the allele itself\, or is it impacted by the state of 
 the other allele?”   Here we apply RNA-seq to female head tissue from 
 progeny of a grid cross of five founder lines from the Drosophila Syntheti
 c Reference Population\, and test how often allele-specific expression lev
 els suggest any inter-allelic interaction. The read counts from the RNA-se
 q experiments can be thought of as pairwise contests between alleles\, wit
 h each allele being involved in several such contests.  To estimate the i
 ntrinsic transcript abundance from each allele\,  we fitted the Bradley-T
 erry model.  This also allowed testing goodness-of-fit to the null hypoth
 esis of no inter-allelic interaction.  The test was applied individually 
 to each of the 8201 genes that had sufficient read depth and polymorphism 
 to accurately call allele-specific expression.  The surprising result was
  that 98% of these genes fitted the model\, implying a lack of interaction
  (i.e. no evidence for transvection). We then explore the possible evoluti
 onary consequences of genes that undergo classical transvection\, where tw
 o different defective alleles complement each other.
LOCATION:Raiffeisen Lecture Hall\, ISTA
ORGANIZER:maria.arias.sutil@ista.ac.at
SUMMARY:Andrew G. Clark: How much does Big-A talk to Little-a?
URL:https://talks-calendar.ista.ac.at/events/4246
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