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TZID:Europe/Vienna
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DTSTART:20170326T030000
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DTSTART:20171029T020000
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DTSTAMP:20260428T111748Z
UID:58ecc78038ea7486028013@ist.ac.at
DTSTART:20170413T160000
DTEND:20170413T170000
DESCRIPTION:Speaker: Jan Brugues\nhosted by Martin Loose\nAbstract: The spi
 ndle is the protein machinery responsible for segregating the genetic mate
 rial into the daughter cells. We now have identified the key molecular pla
 yers that contribute to spindle structure and function\, including motor p
 roteins\, cross-linking proteins\, and proteins that regulate microtubule 
 nucleation and polymerization. However\, it is still not known how the con
 stituent proteins of the spindle self-organize into a into a proper size a
 nd shape. Here\, we used laser ablation to measure the minus ends of monop
 olar spindles—where minus ends remain static—assembled in Xenopus leaevi
 s egg extract  as a proxy to microtubule nucleation. We found that bulk nu
 cleation from a RanGTP-mediated gradient of nucleators alone cannot accoun
 t for the spatial profile of microtubule nucleation in monopoles. Instead\
 , microtubule-stimulated nucleation regulated by the RanGTP gradient expla
 ins the nucleation profile and microtubule density in these structures. Th
 is nucleation mechanism could account for the scaling of spindles with cel
 l volume as observed in early embryogenesis or spindles encapsulated in ex
 tract\, and provides an alternative prediction to previous models based on
  microtubule dynamics (via a limiting pool of tubulin or MAPs).
LOCATION:Mondi Seminar Room 2\, Central Building\, ISTA
ORGANIZER:mloose@ist.ac.at
SUMMARY:Jan Brugues: Mechanisms of microtubule nucleation and size in spind
 les.
URL:https://talks-calendar.ista.ac.at/events/422
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