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DTSTAMP:20260424T113859Z
UID:6267c1da51496183571090@ist.ac.at
DTSTART:20230425T160000
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DESCRIPTION:Speaker: Zoltan Nusser\nhosted by Johann Danzl\nAbstract: Title
 :Understanding the molecular mechanisms underlying postsynaptic target cel
 l type-dependent differences in presynaptic release propertiesAbstract:It 
 has been known for decades that chemical synapses of the CNS show tremendo
 us functional and structural diversity. Understanding the molecular mechan
 isms underlying functional synaptic diversity is a major challenge and has
  been in the focus of our research in the past two decades. A special exam
 ple of synaptic diversity is the so-called postsynaptic target cell type-d
 ependent variations in the presynaptic properties of glutamatergic synapse
 s. The probability of glutamate release from hippocampal pyramidal cell (P
 C) axon terminals that innervate oriens-lacunosum-moleculare (O-LM) intern
 eurons is 10-fold lower that that innervating fast-spiking interneurons (F
 SINs). Our high-resolution immunolocalization experiments revealed that Mu
 nc13-2 has a high density in the active zone of PC axon terminals that inn
 ervate O-LM cells\, but not in FSIN-innervating ones. Using Munc13-2 condi
 tional KO mice and in vitro paired recordings we demonstrated that this Mu
 nc13 isoform plays no detectable role. Pharmacological experiments reveale
 d that the major difference between these synapses is their differential s
 ensitivity to PDBU\, indicating differential priming states of the synapti
 c vesicles probably due to a differential modulation of Munc13-1. Our resu
 lts are consistent with a sequential two state priming model of synaptic t
 ransmission in which PC O-LM synapses\, a 5-fold smaller fraction of the v
 esicles is properly primed when compared to those at PC FSIN synapses. Our
  modelling also predicted that the fusion probability of properly primed v
 esicles differs only two-fold at these synapses\, which is consistent with
  the results of pharmacological\, in vitro Ca2+ imaging and molecular neur
 oanatomical experiments. Our results demonstrate that docked\, but incompl
 etely primed synaptic vesicle limit the output of PC O-LM cell synapses.
LOCATION:Mondi Seminar Room 2\, Central Building\, ISTA
ORGANIZER:mmosiash@ist.ac.at
SUMMARY:Zoltan Nusser: Understanding the molecular mechanisms underlying po
 stsynaptic target cell type-dependent differences in presynaptic release p
 roperties
URL:https://talks-calendar.ista.ac.at/events/4128
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