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DTSTAMP:20260404T145721Z
UID:1681290000@ist.ac.at
DTSTART:20230412T110000
DTEND:20230412T130000
DESCRIPTION:Speaker: Sagar Sridhara\nhosted by Florian Schur\nAbstract: Typ
 e III-A CRISPR-Cas (Csm) system constitutes a multi-component (Csm1-5\, cr
 RNA)\, multipronged ribonucleoprotein effector complex that confers immuni
 ty in bacteria and archaea via foreign-RNA activated and self-RNA inhibite
 d enzymatic activities. Recognition of foreign-RNA triggers indiscriminate
  single-stranded DNase (ssDNase) and cyclic oligonucleotide (cOA) synthesi
 s activities by the signature subunit Csm1. The newly synthesized cOAs tri
 gger downstream activation of ancillary ribonucleases such as Csm6 adding 
 to the collective immune response. We employed cryo-EM\, functional assays
  and comparative cross-linking to study in vivo assembled mesophilic Lacto
 coccus lactis Csm (LlCsm) at various functional states and suggest a possi
 ble role of target RNA-induced protein dynamics in activation of the Csm c
 omplex.Additionally\, we repurposed the LlCsm complex towards the detectio
 n of SARS-CoV-2\, as an off-the-shelf COVID-diagnostic\, by harnessing bot
 h RNA- and transcription-activated dual nucleic acid cleavage activities a
 s well as internal signal amplification allowing virus detection with high
  sensitivity and at multiple settings. Our detection platform named 'MORIA
 RTY' could reliably detect 2000 copies/μl in amplification-free and 60 co
 pies/μl via isothermal amplification within 30 min and diagnosed SARS-C
 oV-2-infected patients in both settings reliably.
LOCATION:Mondi 2\, ISTA
ORGANIZER:
SUMMARY:Sagar Sridhara: Type III-A CRISPR-Cas systems: Structure\, Function
  & Applications towards SARS-CoV-2 diagnosis
URL:https://talks-calendar.ista.ac.at/events/4113
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