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DTSTART:20170326T030000
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DTSTART:20171029T020000
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DTSTAMP:20260428T110248Z
UID:58c27430cf1c9341689834@ist.ac.at
DTSTART:20170331T100000
DTEND:20170331T110000
DESCRIPTION:Speaker: Olivier Thoumine\nhosted by Harald Janovjak\nAbstract:
  To gain insight into the molecular mechanisms by which early neuronal con
 nections mature into excitatory or inhibitory synapses\, we are examining 
 the dynamics\, nanoscale organization\, and signaling mechanisms associate
 d with the adhesion molecule neuroligin-1 (Nlg1).\n\nWe recently developed
  a highly sensitive labeling strategy relying on fluorophore-conjugated mo
 nomeric streptavidin to target Nlg1 carrying a short\, enzymatically bioti
 nylated tag. Using super-resolution imaging techniques including uPAINT\, 
 STED and dSTORM\, we demonstrate specific labeling of Nlg1 at synapses in 
 both dissociated neurons and organotypic slices\, with reduced steric hind
 rance and absence of cross-linking compared to multivalent probes. We are 
 also developing a simulator of single molecule dynamics which allows us to
  quantitatively interpret our live cell imaging experiments (SPT\, FRAP\, 
 FCS). Finally\, we demonstrate an important role of Nlg1 phosphorylation i
 n synapse specification\, focusing on a unique intracellular tyrosine resi
 due (Y782) located in the gephyrin-binding motif (Giannone et al.\, Cell R
 eports 2013). Strikingly\, optogenetic phosphorylation of Nlg1 using a pho
 toactivatable tyrosine kinase\, induces the formation of new dendritic spi
 nes (Letellier et al.\, submitted).\n\nRecent references:\n\nChamma et al.
 \, Nature Protocols 2017\; Chamma et al.\, Nature Comms 2016 \; Garcia et 
 al.\, PNAS 2015 \; Letellier et al.\, Nature Neurosci 2014 \; Czöndör et
  al.\, Nature Comms\, 2013 \; Giannone et al.\, Cell Rep 2013.\n
LOCATION:Mondi Seminar Room 2\, Central Building\, ISTA
ORGANIZER:amally@ist.ac.at
SUMMARY:Olivier Thoumine: Role of adhesion molecule neuroligin-1 in synapto
 genesis probed by single molecule imaging & regulation of tyrosine phospho
 rylation
URL:https://talks-calendar.ista.ac.at/events/372
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