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DTSTART:20170326T030000
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DTSTART:20171029T020000
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DTSTAMP:20260428T204810Z
UID:58c277f659321038743144@ist.ac.at
DTSTART:20170410T110000
DTEND:20170410T120000
DESCRIPTION:Speaker: Guillaume Sandoz\nhosted by Harald Janovjak\nAbstract:
  Ion channels generate the electrical signals with which the nervous syste
 m uses to sense the world\, process information\, create memories and cont
 rol behavior. One of the most diverse family of ion channels\, the K2P cha
 nnels\, serves as a hub for the generation and regulation of the negative 
 resting membrane potential and neuronal excitability. K2P channels also pl
 ay a central role in the response of cells to diverse extracellular and in
 tracellular signals\, such as GPCR signaling\, pH and membrane stretch. Th
 e members of the TREK channel subfamily\, TREK1\, TREK2 and the more dista
 ntly-related TRAAK channel are widely expressed in the nervous system and 
 are involved in several physiological and pathological functions\, includi
 ng pain perception\, depression and PUFA-dependent neuroprotection against
  ischemia.  In this seminar\, I will first describe the molecular basis fo
 r TREK1 and TREK2 channel pH-sensitivity and I will show how these channel
 s can be oppositely regulated by pH. Then\, I will present single-molecule
  imaging data demonstrating that TREK channels can heteromultimerize to in
 crease functional diversity\, and I will discuss the development of a nove
 l optical imaging method to probe interactions of a channel’s isolated in
 tracellular domain with the plasma membrane. In the case of TREK\, we show
 ed that the interaction between the C-terminus and the plasma membrane is 
 involved in the TREK channel gating and the regulation by GPCRs and the an
 tidepressant fluoxetine. Finally\, I will show you how we created light-ga
 ted versions of members of K2P family and an important new method for opto
 genetics\, which we call the photoswitchable conditional subunit method (T
 REK1-PCS)\, which makes it possible to endow native (unmodified) channels 
 with light sensitivity. TREK1-PCS allows us to show that TREK1\, typically
  considered to be only a leak channel\, contributes actively to the hippoc
 ampal GABAB response which breaks with conventional idea that hippocampal 
 GABAB is mediated only by GABAB-GIRK coupling. In addition\, by using this
  tool we have shown how phospholipids act specifically on TREK channels an
 d how small molecules\, such as ethanol\, can specifically modify TREK cha
 nnel functions.
LOCATION:Mondi Seminar Room 3\, Central Building\, ISTA
ORGANIZER:amally@ist.ac.at
SUMMARY:Guillaume Sandoz: Optical probing and optogenetic of TREK channels 
 physiology
URL:https://talks-calendar.ista.ac.at/events/366
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