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DESCRIPTION:Speaker: Romana Schirhagl\nAbstract: Free radical generation pl
 ays a key role in many biological processes including cell communication\,
  immune responses and maturation. However\, radical formation is also a ha
 llmark of ageing and often elevated when cells experience stress. As a res
 ult\, they are important in many different diseases including cancer\, car
 diovascular diseases or viral and bacterial infections. However\, free rad
 icals are short lived and reactive and thus difficult to measure for the s
 tate of the art. Also\, since they occur in low concentrations\, they are 
 difficult to localise. We have circumvented this problem by using a techni
 que which allows nanoscale MRI. This technique makes use of a defect in di
 amond which changes its optical properties based on its magnetic surroundi
 ng. Since optical signals are much easier to detect the method offers orde
 rs of magnitude better sensitivity. Here I would like to show our work in 
 immune cells[1\,2]. In these cells we were able to target nanodiamonds to 
 single mitochondria and measure the metabolic activity of the organelles a
 s well as their stress response[1]. We were able to conduct a similar stud
 y also in primary cells which where harvested from donors[2]. Despite thes
 e donors all being healthy there were drastic differences in how aggressiv
 e their dendritic cells reacted towards a stressor. With these measurement
 s we could confirm that these differences that were also evident in other 
 metabolic parameters also were obvious when observing free radical generat
 ion.Figure 1 – We investigated the stress response of dendritic cells fr
 om donors in an ex-vivo study using NV centers in nanodiamondsReferences[1
 ] Nie\, L.\, Nusantara\, A.C.\, Damle\, V.G.\, Sharmin\, R.\, Evans\, E.P
 .P.\, Hemelaar\, S.R.\, van der Laan\, K.J.\, Li\, R.\, Martinez\, F.P.\, 
 Vedelaar\, T. and Chipaux\, M.\, 2021. Quantum monitoring of cellular meta
 bolic activities in single mitochondria. Science advances\, 7(21)\, p.eabf
 0573.[2] Nie\, L.\, Nusantara\, A.C.\, Damle\, V.G.\, Baranov\, M.V.\, Chi
 paux\, M.\, Reyes-San-Martin\, C.\, Hamoh\, T.\, Epperla\, C.P.\, Guricova
 \, M.\, Cigler\, P. and Van Den Bogaart\, G.\, 2021. Quantum sensing of fr
 ee radicals in primary human dendritic cells. Nano letters.
LOCATION:Online\, ISTA
ORGANIZER:arinya.eller@ist.ac.at
SUMMARY:Romana Schirhagl: Relaxometry for measuring free radical generation
  in living cells
URL:https://talks-calendar.ista.ac.at/events/3447
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