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DTSTART:20170326T030000
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DTSTART:20161030T020000
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DTSTAMP:20260428T235400Z
UID:58a6b45adcbea038905072@ist.ac.at
DTSTART:20170223T164500
DTEND:20170223T173000
DESCRIPTION:Speaker: Karuna Sampath\nhosted by Carl-Philipp Heisenberg\nAbs
 tract: During development gene expression is controlled by regulatory mech
 anisms that act at the level of DNA and/or RNA. We find that a 3' un-trans
 lated region (3'UTR) RNA element that we previously identified in zebrafis
 h nodal-related1/squint (sqt)\, is shared by multiple components of the No
 dal signaling pathway: ligand\, receptor\, inhibitor and transcriptional e
 ffector. RNA localization assays in early zebrafish embryos using fluoresc
 ent reporters show that the 3’UTR RNA elements in lefty 1 and lefty2 inh
 ibitor RNAs\, acvr2 receptor RNA and to a lesser extent\, smad2 effector R
 NA\, behave similarly to sqt/nodal ligand RNA. The RNA-binding protein (RB
 P) and translational repressor\, Y-box binding protein 1 (Ybx1) binds to t
 he 3’UTR element and translation pre-initiation complex proteins to prev
 ent translation. Whereas ybx1 mutant embryos show premature Nodal/Sqt tran
 slation and gain-of-Nodal signaling\, compound ybx1\;sqt mutants manifest 
 premature and elevated Lefty protein expression and loss-of-Nodal signalin
 g. These findings demonstrate translational co-regulation of multiple comp
 onents of a developmental pathway by a shared RNA element/RBP “regulon".
  This module regulates NODAL RNA in human cells as well\, suggesting broad
 er conservation.\nTo understand the basis of Ybx1 RBP/RNA element recognit
 ion\, we performed computational analysis of the zebrafish transcriptome. 
 We identified >800 candidate RNAs harboring the 3’UTR element\, of which
  many encode components of signaling pathways (e.g.\, midkine\, FGF) or fa
 ctors that function in the same biological process (e.g.\, metabolism\, ge
 rm-line development). To determine the role of the putative elements\, we 
 are establishing high-throughput assays for localization and translation i
 n zebrafish embryos. Our findings suggest that the RNA regulatory module t
 hat we first identified in nodal RNA might be used for co-regulation of mu
 ltiple signaling pathways and sets of functionally connected genes during 
 development.\n \n
LOCATION:Experimental Biology Room (I04.2OG - LAB)\, ISTA
ORGANIZER:lalesch@ist.ac.at
SUMMARY:Karuna Sampath: Coordinate Regulation of Development by a Shared RN
 A Element
URL:https://talks-calendar.ista.ac.at/events/326
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