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DTSTART:20170326T030000
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DTSTART:20161030T020000
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DTSTAMP:20260429T024522Z
UID:5880e6bde1d12490000910@ist.ac.at
DTSTART:20170125T133000
DTEND:20170125T144500
DESCRIPTION:Speaker: John Marko\nhosted by Calin Guet\nAbstract: I will dis
 cuss micromechanics experiments that our group has used to analyze protein
 -DNA interactions and chromosome organization. In single-DNA experiments w
 e have found that a feature of protein-DNA complexes is that their dissoci
 ation rates can depend strikingly on bulk solution concentrations of other
  proteins and DNA segments\; I will describe experiments which demonstrate
  this effect\, which can involve tens-fold changes in off-rates with submi
 cromolar changes in solution concentrations. Second\, I will discuss exper
 iments aimed at analyzing large-scale human chromosome structure\; we isol
 ate metaphase chromosomes\, which in their native form behave as remarkabl
 y elastic networks of chromatin. Exposure to DNA-cutting restriction enzym
 es completely eliminates this elasticity\, indicating that there is not a 
 mechanically contiguous protein "scaffold" from which the chromosome gains
  its stability. I will show results of siRNA experiments indicating that d
 epletion of condensin proteins leads to destabilization of chromosome mech
 anics\, indicating condensin's role as the major chromatin "cross-linker" 
 in metaphase chromosomes. Finally I will discuss similar experiments on hu
 man G1 nuclei\, where we use genetic and chemical modifications to separat
 e the contributions of the nuclear lamina and chromatin to the mechanical 
 stiffness of the nucleus as a whole.
LOCATION:Oberbank Ballroom\, Central Building\, ISTA
ORGANIZER:lalesch@ist.ac.at
SUMMARY:John Marko: Micromechanical study of protein-DNA interactions and c
 hromosomes
URL:https://talks-calendar.ista.ac.at/events/267
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