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DTSTAMP:20260404T015842Z
UID:1582297200@ist.ac.at
DTSTART:20200221T160000
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DESCRIPTION:Speaker: Stefan Hell\nhosted by Johann Danzl\nAbstract: I will 
 show how an in-depth description of the basic principles of diffraction-un
 limited fluorescence microscopy (nanoscopy) [1-3] has spawned a new powerf
 ul superresolution concept\, namely MINFLUX nanoscopy [4]. MINFLUX utilize
 s a local excitation intensity minimum (of a doughnut or a standing wave) 
 that is targeted like a probe in order to localize the fluorescent molecul
 e to be registered. In combination with single-molecule switching for sequ
 ential registration\, MINFLUX [4-6] has obtained the ultimate (super)resol
 ution: the size of a molecule. MINFLUX nanoscopy\, providing 1–3 nanomet
 er resolution in fixed and living cells\, is presently being established f
 or routine fluorescence imaging at the highest\, molecular-size resolution
  levels. Relying on fewer detected photons than popular camera-based local
 ization\, MINFLUX nanoscopy is poised to open a new chapter in the imaging
  of protein complexes and distributions in fixed and living cells.[1]   
 Hell\, S.W.\, Wichmann\, J. Breaking the diffraction resolution limit by s
 timulated emission: stimulated-emission-depletion fluorescence microscopy.
  Opt. Lett. 19\, 780-782 (1994).[2]   Hell\, S.W. Far-Field Optical Na
 noscopy. Science 316\, 1153-1158 (2007).[3]   Hell\, S.W. Microscopy and
  its focal switch. Nat. Methods 6\, 24-32 (2009).[4]   Balzarotti\, F.\,
  Eilers\, Y.\, Gwosch\, K. C.\, Gynnå\, A. H.\, Westphal\, V.\, Stefani\,
  F. D.\, Elf\, J.\, Hell\, S.W. Nanometer resolution imaging and tracking 
 of fluorescent molecules with minimal photon fluxes. Science 355\, 606-612
  (2017).[5]  Eilers\, Y.\, Ta\, H.\, Gwosch\, K. C.\, Balzarotti\, F.\, 
 Hell\, S. W. MINFLUX monitors rapid molecular jumps with superior spatiote
 mporal resolution. PNAS 115\, 6117-6122 (2018).[6]   Gwosch\, K. C.\, Pa
 pe\, J. K.\, Balzarotti\, F.\, Hoess\, P.\, Ellenberg\, J.\, Ries\, J.\, H
 ell\, S. W. MINFLUX nanoscopy delivers multicolor nanometer 3D-resolution 
 in (living) cells.  (bioRxiv\, doi: https://doi.org/10.1101/734251)
LOCATION:Raiffeisen Lecture Hall\, ISTA
ORGANIZER:arinya.eller@ist.ac.at
SUMMARY:Stefan Hell: MINFLUX nanoscopy and related matters
URL:https://talks-calendar.ista.ac.at/events/2659
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