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DTSTART:20200329T030000
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DTSTART:20191027T020000
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DTSTAMP:20260405T161918Z
UID:5dd2b586363bd072500222@ist.ac.at
DTSTART:20191127T110000
DTEND:20191127T120000
DESCRIPTION:Speaker: Johanna Fischer\nhosted by Fyodor Kondrashov\nAbstract
 : High fidelity orchestration of gene expression during development requir
 es precise temporal and spatial control mechanisms. In the first part of t
 his talk this phenomenon will be explored in the light of liver developmen
 t. The generation of a hepatoblast specific transgenic zebrafish line\, wh
 ich enables specific mis  expression in hepatoblasts will be elucidated. D
 ownstream applications for this transgenic line\, which lead to better und
 erstanding of livermorphogenesis will be introduced. In the second part po
 ssible mechanisms of transcriptional regulation of gene expression at a pa
 rticularly interesting class of developmental gene loci\, which are rich i
 n conserved non - coding elements (CNEs). Currently their role in gene reg
 ulation remains unknown. So far 35 CNE clusters have been identified\, whe
 re two or more developmental genes are embedded within the cluster\, which
  are divided via a Topologically Associated Domain (TAD) boundary. There I
  hypothesized that this observation could be (1) coincidence\, (2) sharing
  functional parts of the non  coding genome and (3) co-regulation. In orde
 r to test whether the genes are co-expressed I performed a gene expression
  analysis in three different neuronal progenitor populations of 9 candidat
 e loci. I observed that EphA4 and Pax3 showed evidence of co-expression in
  dorsal forebrain progenitors using qPCR analysis\, which is a form of co-
 regulation. In the other candidate loci and other neuronal progenitor cell
  types no co-expression could be detected\, however we cannot exclude that
  there is co-repression. Subsequently I studied the role of CNEs in gene r
 egulation using a chromosome conformation capture approach. I used neurona
 l stem cells as a model. I hypothesized that either the CNEs interact to f
 orm the higher order chromatin structures\, or that a single CNE preferent
 ially interact in proximity with the promoter region to regulate gene expr
 ession. Eventually\, I identified candidate CNEs\, which could be regulato
 ry elements to regulate gene expression in neuronal stem cells. All togeth
 er these findings may contribute to a better understanding of the orchestr
 ation of gene expression during development.
LOCATION:Seminar Room\, Lab Building East\, ISTA
ORGANIZER:amally@ist.ac.at
SUMMARY:Johanna Fischer: From exploring liver organogenesis to chromosome a
 rchitecture in neuronal stem cells
URL:https://talks-calendar.ista.ac.at/events/2425
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