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DTSTART:20190331T030000
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DTSTART:20191027T020000
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DTSTAMP:20260404T110038Z
UID:5d230259610c7850100608@ist.ac.at
DTSTART:20190709T150000
DTEND:20190709T160000
DESCRIPTION:Speaker: Rishita Changede\nhosted by Michael Sixt\nAbstract: Ce
 ll matrix adhesions attach the cell to the extracellular matrix\, sense it
 s force and geometry and convert that information into biochemical signals
  so that the cell can mount an appropriate response. How do fibroblasts pr
 esent in connective tissues of various stiffness respond correctly to subs
 trate stiffness and how are these signals converted into biochemical signa
 ls? To get insights into these questions\, we combines super resolution mi
 croscopy and live cell microscopy with nanopatterning of the substrates. U
 sing supported lipid bilayers\, we discovered that integrin nanoclusters o
 n integrins are laid out on substrates of all rigidities as a starting poi
 nt for formation of cell matrix adhesions. They form as a first response t
 o sensing the biochemical ligand and help the cell to sense the substrate 
 rigidity. Using nanopatterned fiber mimetic lines functionalized with RGD\
 , we discovered that assembly of these nanoclusters is sensitive to substr
 ate geometry wherein they assemble only on two dimensional substrates but 
 not on one dimensional susbtrates. As these nanoclusters bridge the thin f
 iber mimetic substrates by clustering both ligand bound and unbound (albei
 t activated) integrins. Further\, we discovered that these early nanoclust
 ers serve as differential signalling platforms wherein critical signals re
 quired for cell survival and growth including talin cleavage and ligand in
 dependent EGFR signalling is activated specifically on rigid substrates. M
 oreover\, we showed that mature adhesions are aggregates of integrin nanoc
 lusters and these clusters serve as signalling platforms to bring about fu
 nction. Taken together these results indicated that nanoclusters of integr
 ins formed on 2D substrates are key to downstream adhesion signalling need
 ed for survival\, motility and growth.
LOCATION:Mondi Seminar Room 1\, Central Building\, ISTA
ORGANIZER:astawick@ist.ac.at
SUMMARY:Rishita Changede: Modular integrin nanoclusters are signalling unit
 s of cell matrix adhesions.
URL:https://talks-calendar.ista.ac.at/events/2029
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