BEGIN:VCALENDAR VERSION:2.0 PRODID:icalendar-ruby CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VTIMEZONE TZID:Europe/Vienna BEGIN:DAYLIGHT DTSTART:20190331T030000 TZOFFSETFROM:+0100 TZOFFSETTO:+0200 RRULE:FREQ=YEARLY;BYDAY=-1SU;BYMONTH=3 TZNAME:CEST END:DAYLIGHT BEGIN:STANDARD DTSTART:20191027T020000 TZOFFSETFROM:+0200 TZOFFSETTO:+0100 RRULE:FREQ=YEARLY;BYDAY=-1SU;BYMONTH=10 TZNAME:CET END:STANDARD END:VTIMEZONE BEGIN:VEVENT DTSTAMP:20260403T220514Z UID:5ce7ce2712f66896116695@ist.ac.at DTSTART:20190603T110000 DTEND:20190603T121500 DESCRIPTION:Speaker: Laura Lorenzo Orts\nhosted by Carl-Philipp Heisenberg\ nAbstract: Polyphosphates (polyPs) are linear polymers formed by orthophos phate subunits linked by high-energy phosphoanhydride bonds. While polyPs exist in all domains of life (from bacteria to humans)\, their presence in plants is not yet confirmed. We identified plant proteins with structural homology to polyP-metabolizing enzymes. One of them\, TTM3\, cleaves trip olyphosphate molecules into pyrophosphate and orthophosphate. My work reve als that TTM3 is expressed together with the CELL DIVISION CYCLE PROTEIN 2 6 (CDC26) from a bona fide bicistronic mRNA that is conserved in the entir e plant lineage. CDC26 is part of the plant anaphase promoting complex and its deletion impairs embryo development. While TTM3 enzymatic activity se ems dispensable\, TTM3 translation coordinates CDC26 expression by targeti ng the transcript into polysomes. Hence\, the bicistronic configuration of the CDC26-TTM3 mRNA allows fine-tuning of CDC26 expression and suggests a functional connection between polyP metabolism and the cell cycle. Furthe rmore\, a protein from Ricinus communis was found to harbor a domain of un known function named CHAD. In bacteria\, CHAD domains are often found in o perons encoding polyP-metabolizing enzymes. My work demonstrates that CHAD from all domains of life bind polyPs with high affinity and specificity. Interestingly\, CHAD localizes in the nucleus and nucleolus of plant cells \, suggesting the existence of a polyP pool in these compartments. Based o n our biochemical and structural analysis\, CHAD may be used as a molecula r tool to detect polyPs in cells and tissues. LOCATION:Meeting room 2nd floor / Bertalanffy Bldg. (I04.2OG - LAB)\, ISTA ORGANIZER:lalesch@ist.ac.at SUMMARY:Laura Lorenzo Orts: "\;Inorganic polyphosphates in plants: sens or domains\, metabolic enzymes... and an unsuspected bicistronic mRNA" \;. URL:https://talks-calendar.ista.ac.at/events/1993 END:VEVENT END:VCALENDAR