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DTSTART:20190331T030000
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DTSTART:20181028T020000
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DTSTAMP:20260405T054125Z
UID:5c64347fd2843154882369@ist.ac.at
DTSTART:20190307T100000
DTEND:20190307T110000
DESCRIPTION:Speaker: Kikue Tachibana\nhosted by Simon Hippenmeyer\nAbstract
 : Totipotency\, the potential of a cell to generate all cell types and a n
 ew organism\, is achieved when egg and sperm fuse to form the one-cell zyg
 ote. How chromatin is epigenetically reprogrammed to totipotency within ho
 urs after fertilisation is a central question in biology. We aim to addres
 s this by investigating the mechanisms of reprogramming and spatial chroma
 tin reorganisation in mammalian zygotes. The zygotic paternal genome is de
 methylated by a mechanism that was proposed to involve DNA breaks. We prov
 ide genetic evidence for a requirement of DNA repair in reprogramming and 
 discovered that zygotic reprogramming is monitored by a surveillance. To s
 tudy how the totipotent state is generated\, we pioneered single-nucleus H
 i-C that enables the genome-wide quantification of chromatin structure in 
 isolated maternal and paternal zygotic nuclei. Both genomes are organized 
 into loops and topologically associating domains that strictly depend on c
 ohesin. Unexpectedly\, we discovered that the maternal genome lacks strong
  compartments. An understanding of this zygotic chromatin ground state cou
 ld potentially provide insights into reprogramming cells to a state of tot
 ipotency.
LOCATION:Mondi Seminar Room 2\, Central Building\, ISTA
ORGANIZER:tguggenb@ist.ac.at
SUMMARY:Kikue Tachibana: How the genome is reorganized during zygotic repro
 gramming to totipotency
URL:https://talks-calendar.ista.ac.at/events/1809
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