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TZID:Europe/Vienna
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DTSTART:20180325T030000
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TZOFFSETTO:+0200
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DTSTART:20181028T020000
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BEGIN:VEVENT
DTSTAMP:20260406T024005Z
UID:5bb31ed4a03e0759071639@ist.ac.at
DTSTART:20181004T140000
DTEND:20181004T150000
DESCRIPTION:Speaker: Alexander Jesacher\nhosted by Johann Georg Danzl\nAbst
 ract: Localization microscopy is based on taking many images of an object\
 , each containing a sparse subset of active molecules that can be individu
 ally localized. The entire data set represents the object at very fine spa
 tial details down to the nanometer range.While localizing within the focal
  plane is relatively straightforward\, retrieving depth information is mor
 e challenging due to the cigar-shaped point response of microscopes. Sever
 al methods which address this problem have been developed over the years.I
 n my talk I will provide a brief introduction of 3D localization methods a
 nd put an emphasis on Supercritical Angle Fluorescence (SAF) microscopy\, 
 which enables the accurate axial localization of light emitters in close v
 icinity to the coverslip by exploiting near-field coupling.I will further 
 address the challenges posed by unknown optical aberrations in the imaging
  system and give an outlook on the use of machine learning algorithms to p
 rocess the large amounts of data which are often collected in localization
  microscopy.
LOCATION:Seminar Room\, Lab Building East\, ISTA
ORGANIZER:rsix@ist.ac.at
SUMMARY:Alexander Jesacher: 3D Localization of Molecules close to dielectri
 c Surfaces
URL:https://talks-calendar.ista.ac.at/events/1467
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