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DTSTART:20180325T030000
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DTSTAMP:20260404T082518Z
UID:5b8ce36e6fbe2829306208@ist.ac.at
DTSTART:20180925T140000
DTEND:20180925T150000
DESCRIPTION:Speaker: U. Valentin Nägerl\nhosted by Johann Georg Danzl\nAbs
 tract: The advent of super-resolution microscopy has created unprecedented
  opportunities to study the mammalian central nervous system\, which is do
 minated by anatomical structures whose nanoscale dimensions critically inf
 luence their biophysical properties and physiological functions. I will pr
 esent our recent methodological advances 1) to analyze dendritic spines in
  the hippocampus in vivo\; 2) to visualize the extracellular space (ECS) o
 f the brain and 3) to reveal the morphological structure and molecular arr
 angement of adhesive structures and synapses in live cells at the nanoscal
 e level.We established chronic in vivo super-resolution imaging of dendrit
 ic spines in the hippocampus\, based on an upright 2P-STED microscope equi
 pped with a long working distance objective and hippocampal window to reac
 h this deeply embedded structure. We measured spine density on pyramidal n
 eurons in the CA1 area and determined spine turnover by repetitive imaging
 . Spine density was two times higher than reported by conventional 2P micr
 oscopy\, and around 40% of all spines turned over within 4 days\, indicati
 ng a high level of structural remodeling of synaptic circuits in a brain a
 rea closely associated with memory functions.We combined 3D-STED microscop
 y and fluorescent labeling of the extracellular fluid to develop super-res
 olution shadow imaging (SUSHI) of brain ECS in living brain slices. SUSHI 
 enables quantitative analysis of ECS structure and at the same time produc
 es sharp negative images of all cellular structures\, providing an unbiase
 d view of unlabeled brain cells with respect to their complete anatomical 
 context in a live tissue setting. As a straight-forward application of sup
 er-resolution microscopy\, SUSHI represents a paradigm shift in cellular b
 io-imaging because it enables panoramic imaging of complex biological tiss
 ues with nanoscale spatial resolution.Current super-resolution microscopes
  are proficient at collecting either single molecule or morphological info
 rmation\, but not both. I will present a new super-resolution platform tha
 t permits correlative single molecule imaging and STED microscopy in livin
 g cells. We demonstrate that this multi-modal approach can give access to 
 both kinds of data by revealing on a nanometer spatial scale protein local
 ization and dynamics and cellular morphology using dendritic spines and gr
 owth cones of primary neuronal cultures as experimental preparation.
LOCATION:Big Seminar room Ground floor / Office Bldg West (I21.EG.101)\, IS
 TA
ORGANIZER:rsix@ist.ac.at
SUMMARY:U. Valentin Nägerl: Super-resolution microscopy for neuroscience: 
 new approaches & applications
URL:https://talks-calendar.ista.ac.at/events/1388
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