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DTSTAMP:20260404T015838Z
UID:5b0bc1a950c97015283762@ist.ac.at
DTSTART:20180607T100000
DTEND:20180607T110000
DESCRIPTION:Speaker: Florian Baumgart\nhosted by Johann Georg Danzl\nAbstra
 ct: Multiple labs have used single molecule localization microscopy (SMLM)
  to report the presence of protein nanoclusters at the T cell plasma membr
 ane. In particular\, the T cell receptor (TCR) complex was suggested to be
  non-randomly organized in resting T cells (1\, 2). This finding has led t
 o a variety of models to explain highly specific and sensitive antigen rec
 ognition\, which culminates in T cell activation. In our lab\, we have pre
 viously found that in SMLM experiments\, characteristic blinking and resul
 ting overcounting of fluorescent proteins and organic dyes may be easily m
 isinterpreted as molecular clusters. As a possible solution to the problem
 \, we developed a method to discriminate between true protein clusters and
  multiple counts of the same dye molecules (3).Here\, we applied this meth
 od to study the organization of the TCR complex in the plasma membrane of 
 resting and activated T cells. While there was clear evidence for the form
 ation of clusters upon activation\, no indication of non-homogenous distri
 bution could be obtained under resting conditions. In independent experime
 nts\, we confirmed our observations with STED microscopy\, yielding simila
 r results. Overall\, our data do not support the view of protein nanoclust
 ering being a general ordering principle at the T cell plasma membrane.(1)
  Lillemeier et at. Nat Immunol. 2010 Jan\;11(1):90-6.(2) Pageon et al. Pro
 c Natl Acad Sci U S A. 2016 Sep 13\;113(37).(3) Baumgart et al. Nat Meth. 
 2016\;13(8):661-4.
LOCATION:Mondi Seminar Room 3\, Central Building\, ISTA
ORGANIZER:rsix@ist.ac.at
SUMMARY:Florian Baumgart: Probing the nanoscale organization of the T cell 
 receptor in the immunological synapse
URL:https://talks-calendar.ista.ac.at/events/1261
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