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DTSTART:20180325T030000
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DTSTART:20181028T020000
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DTSTAMP:20260404T065709Z
UID:5ac4d5838bae4161315304@ist.ac.at
DTSTART:20180416T110000
DTEND:20180416T120000
DESCRIPTION:Speaker: Clyde Pinto\nhosted by Carl-Philipp Heisenberg\nAbstra
 ct: Epithelia have apical surface structures that aid in their function. T
 hese include intestinal microvilli that are important for absorption and a
 ctin microridges that likely play a role in glycocalyx organization and ab
 rasion resistance in non-keratinizing stratified squamous epithelia. Actin
  microridges are actin protrusions that have a maze-like organization. How
  such laterally-long actin protrusions are built remains unclear\, with se
 emingly contradictory evidence indicating that either multiple bundles or 
 a network of actin form this structure and a hypothesis suggesting that it
  is formed from multiple microvilli fused side to side. Using light and el
 ectron microscopy in the zebrafish periderm we show that microridges exhib
 it molecular and structural similarities to the leading edge of migrating 
 cells\, such as the localization of the Arp2/3 complex\, WASp-like protein
 \, filamin\, cofilin and alpha-actinin and the organization of actin in a 
 network of filaments with no obvious bundles present. We also find that th
 e underlying terminal web cytoskeleton is arranged parallel to the membran
 e resembling the lamella of motile cells. Consequently\, we propose that m
 icroridges can be thought of as multiple leading edge-like protrusions on 
 the apical surface of a polarized epithelial sheet. In another part of my 
 talk\, I will present evidence that the function of myosin Vb is essential
  for the maintenance of normal peridermal microridges as well as that of m
 icrovilli in enterocytes. Furthermore\, I will show that in the absence of
  myosin Vb function zebrafish enterocytes exhibit cellular attributes of m
 icrovillus inclusion disease\, a fatal enteropathy in human infants. Overa
 ll\, my work reveals how cells form laterally-long actin-based protrusions
  and establishes a zebrafish model to study the pathophysiology of microvi
 llus inclusion disease.
LOCATION:Meeting room 2nd floor / Bertalanffy Bldg. (I04.2OG - LAB)\, ISTA
ORGANIZER:lalesch@ist.ac.at
SUMMARY:Clyde Pinto: Epithelial apical actin protrusions: using zebrafish t
 o understand them in development and disease
URL:https://talks-calendar.ista.ac.at/events/1203
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