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DTSTART:20180325T030000
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DTSTART:20171029T020000
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DTSTAMP:20260405T192554Z
UID:5a5c69bfb0128124606550@ist.ac.at
DTSTART:20180319T100000
DTEND:20180319T110000
DESCRIPTION:Speaker: Nikolaus Grigorieff\nhosted by Leonid Sazanov\nAbstrac
 t: In the past five years\, single-particle electron cryo-microscopy (cryo
 -EM) has evolved into a routine technique in structural biology that deliv
 ers high-resolution images of molecules and their assemblies\, on par with
  X-ray crystallography. Unlike crystallography\, single-particle cryo-EM c
 an also visualize many different conformational states present in a single
  sample\, providing snapshots of the functional cycle of the imaged molecu
 les and assemblies. I will provide examples of what can be done today and 
 discus challenges for the future.Most molecules and assemblies function wi
 thin the larger context of a cell\, which can be visualized by electron to
 mography (ET). Despite groundbreaking development of sample preparation an
 d image processing techniques\, cryo-ET is still limited to about 2 nm res
 olution unless averaging techniques can be applied. At this resolution\, i
 t is difficult to infer the chemical interactions between the molecules th
 at underlie cellular mechanisms. I will describe a cryo-EM approach that a
 llows the precise placement of molecules of known structure into the conte
 xt of cellular environments\, thereby addressing one of the current limita
 tions of tomography.
LOCATION:Big Seminar room Ground floor / Office Bldg West (I21.EG.101)\, IS
 TA
ORGANIZER:pdelreal@ist.ac.at
SUMMARY:Nikolaus Grigorieff: After the Resolution Revolution – Quo Vadis\,
  Cryo-EM?
URL:https://talks-calendar.ista.ac.at/events/1102
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