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DTSTART:20180325T030000
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DTSTART:20171029T020000
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DTSTAMP:20260424T143456Z
UID:5a61f2422c423409510877@ist.ac.at
DTSTART:20180207T110000
DTEND:20180207T120000
DESCRIPTION:Speaker: John LaCava\nhosted by Fyodor Kondrashov\nAbstract: LI
 NE-1 (L1) retrotransposons are catalysts of evolution and disease whose se
 quences constitute a significant proportion of the human genome\, propagat
 ing via a copy-and-paste mechanism through an RNA intermediate. Despite tr
 emendous influence on genome composition\, L1 RNAs only encode two protein
 s (creatively termed ORF1p and ORF2p). Consequently\, in vivo\, L1 macromo
 lecules engage a combination of permissive host factors that are essential
  to their lifecycle as well as repressive factors that constitute host def
 enses against L1's deleterious activity. In an attempt to better understan
 d the molecular biology of L1\, we have characterized host proteins associ
 ated with human L1 retrotransposons\, as expressed in cell culture\, using
  a combination of techniques including metabolic labeling and affinity pro
 teomics. In this seminar I will primarily discuss our production and use o
 f these data to create a multi-dimensional interactomic characterization o
 f affinity isolated L1s\; an interactive discussion with the audience conc
 erning general and technical aspects of affinity proteomic experimental de
 sign is encouraged.General background(1) Taylor\, M. S. et al. Affinity Pr
 oteomics Reveals Human Host Factors Implicated in Discrete Stages of LINE-
 1 Retrotransposition. Cell 155\, 10341048 (2013).(2) LaCava\, J. et al. Af
 finity proteomics to study endogenous protein complexes: Pointers\, pitfal
 ls\, preferences and perspectives. BioTechniques 58\, 103119 (2015).(3) Ta
 ylor\, M. S. et al. Dissection of affinity captured LINE-1 macromolecular 
 complexes. Elife 7\, e30094 (2018).
LOCATION:Mondi Seminar Room 3\, Central Building\, ISTA
ORGANIZER:amally@ist.ac.at
SUMMARY:John LaCava: Exploring the interactome of a human genetic parasite:
  affinity proteomics versus LINE-1 retrotransposons
URL:https://talks-calendar.ista.ac.at/events/1056
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